Multiresidue method for the determination of sulfonamides in pork tissue.
Long A.R. | Hsieh L.C. | Malbrough M.S. | Short C.R. | Barker S.A.
A multiresidue technique for the isolation and liquid chromatographic determination of eight sulfonamides from pork muscle tissue is described. Sulfanilamide, sulfathiazole, sulfadiazine, sulfamerazine, sulfamethazine, sulfamethoxazole, sulfisoxazole, and sulfadimethoxine standards were fortified into pork tissue (0.5 g) and blended with C18 (octadecylsilyl-derivatized silica) packing material (2 g). The blended C18/muscle tissue matrix was used to prepare a column that was washed with hexane (8 mL). Sulfonamides were eluted with methylene chloride (8 mL). Sample extracts contained sulfonamide analytes (62.5-2000 ng/g) that were free from interfering compounds when examined by HPLC utilizing photodiode array detection at 270 nm. Correlation coefficients of standard curves of sulfonamides isolated from fortified pork tissue ranged from 0.994 (+/- 0.006) to 0.999 (+/- 0.001). Percentage recoveries (70.4 - 95.8%) and intra- (3.46 - 6.17%) and interassay variabilities (4.04 - 14.05%) for the eight sulfonamides were indicative of a suitable quantitative method for the analysis of these compounds in a muscle tissue matrix.
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