Effect of interaction between variety and culture medium on induction capacity of adventitious bud from cotyledon explant in pepper, Capsicum annuum L.
1991
Kato K. | Suzuki Y. | Nomura T. | Hayashi K.
Potentiality of four different culture media (see table 1) was evaluated to find out the best medium for inducing adventitious buds from cotyledon explants in pepper, using 33 varieties (see table 4) representing a wide genetic variation. Depending on varieties and culture media, a wide variation from 0.03 to 21.6was observed in culture efficiency which represented the average number of adventitious buds induced from one inoculated explant. The analysis of variance showed that culture efficiency averaged for 33 varieties was not different among the media (tables 2 and 3). It was thus concluded that all of the culture media possessed the same potentiality for inducing adventitious buds. On the contrary, a wide varietal variation ranged from 2.2 to 14.0 was observed in culture efficiency averaged for four culture media (tables 3 and 4). This indicated the importance of the choice of variety used for the culture. The interaction between varieties and culture media also proved statistically significant by the analysis of variance (table 3), clearly showing that the favorable medium was different among varieties (table 4 and fig. 2). It was also suggested that the use of different types of varieties partly resulted in the establishment of four different media (table 6). Culture efficiency attained on the most favorable medium for each variety was different among varieties and ranged from 3.7 to 21.6 from practical point of view, therefore, in vitro mass propagation proved to be possible for all of the pepper varieties tested. The analysis of the relationship between variety group and culture response demonstrated that the most favorable medium seemed different between variety groups in red pepper (table 5); "A" medium for "Yatsubusa" group, "B" medium for "Enomi" and "Fushimi" groups, and "C" medium for "Takanotsume" group.
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