Characterization of biosurfactant from Saccharomyces cerevisiae 2031 and evaluation of emulsification activity for potential application in bioremediation
Alcantara, V.A. | Pajares, I.G. | Simbahan, J.F., Philippines Univ. Los Baños, College, Laguna (Philippines). National Inst. of Molecular Biology and Biotechnology | Villarante, N.R. | Rubio, M.L.D., Philippines Univ. Manila, Manila (Philippines). Dept. of Physical Sciences and Mathematics
The boisurfactant isolated from Saccharomyces cerevisiae 2031 using glucose and waste cooking oil as additional carbon sources was characterized. Its emulsification activity was evaluated for potential application in bioremediation. Heat treatment for the S.cerevisiae 203 cells was done to solubilize the biosurfactant. The extracted biosurfactant was then partially purified by acetone precipitation. Qualitative test using the drop collapsed technique indicated presence of a biosurfactant with emulsion-stabilizing property. Biochemical analysis of the partially purified biosurfactant(PPBS) revealed that it is a protein-polysaccharide complex, the typical composition of mannoproteins. Fourier transform infrared(FTIR) spectroscopy further confirmed the presence of these macromolecules. Emulsification properties of biosurfactant revealed that it is not affected by a wide range of environmental conditions such as pH(2-12), temperature(4-70 deg C) and salt concentration(2-10%), thus making it useful for various applications. It can also effectively emulsify different commercial oils, aliphatic and aromatic hydrocarbons, organic solvents and waste cooking oil, also making it suitable for bioremediation of lipid-based and petroleum-based contaminated wastes.
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