Ultramicrostructures descriptions of Musa pollen
2007
Nurulashikin, A.H. | Ghizan, S. | Abdulla, N.A.P. | Zakaria, W., Universiti Putra Malaysia, 43400, Serdang, Selangor (Malaysia). Dept. of Crop Science
Phylogenetics of Musa has been studied by using AFLP and chloroplast DNA (cpDNA). However, phylogenetic tree or dendrogram will alter when there is addition of new genotype in the analysis. Therefore, phylogenetic analysis combined with morphological characters will strengthen the analysis. In this study, pollen grains of 41 genotypes of Musa distributed in Malaysia were examined by light and Scanning Electron Microscopy to study the morphological character. The results from this study will be combined with the phytogenetic study of the same genotypes of Musa. The objective of this study was to study the pollen morphology variation between these genotypes in Malaysia. The pollen samples were obtained mostly from fresh collected specimens from Musa collection at Ladang 2, Universiti Putra Malaysia. Fresh Musa flowers were fixed in FAA solution prior to microscopy examination. Pollen grains were examined by using light microscopy (LM) to observe the general shape and aperture number. This study followed the standard method described by Erdtman (1969). Then, Scanning Electron Microscopy (SEM) was used to examine the measurements of polar axis (P) and equatorial diameter (E) from 10 grains per sample to calculate the P/E ratio. P/E ratio will determine the pollen shape. From the SEM, aperture type and surface ornamentation will also be obtained. For scanning electron microscopy (SEM), it followed the protocol explained by Davies (1999) with some modification. From this study, classification of genotypes will be compared to further phytogenetic studies.
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