Development of a screening method for isolation and endophytic bacteria from sugarcane
2009
Rattanakreetakul, C. | Bunjoedchoedchu, R. | Lersrutaiyotin, R.
Endophytic bacteria are a group of bacteria living in plant tissues without harm for the host plant. Bacteria were found from surface-disinfected plant tissue or internal plant tissue extraction. In several crops, endophytic bacteria have been found on their beneficial effects such as plant growth and health production, nitrogen fixation, phytohormones production, antifungal compounds production which tended to induce systemic resistance of plant. In sugarcane, endophytic bacteria become a major concern on enhanced plant on growth and yield. The study focused on isolation and screening method of endophytic bacteria from sugarcane tissue. Two extraction methods were studied as 1) sugarcane tissue grinding and 2) sugarcane tissue centrifuge. Different media were used to isolate bacteria, nutrient agar and modified Czapek solution agar (Czapek solution agar with 20% sucrose plus 250 ppm bromthymol blue). Extraction of bacteria through centrifuge and isolation with modified Czapek agar was showed a potential for screening of endophytic bacteria from sugarcane tissue. There was minimized extraction time used in each sample with similar result of colony type and acid production. After the selection, bacteria were determined on their ethylene production from acetylene using gas chromatography. Bacteria were divided to group by acid production data, growth ability on high sucrose content and acetylene transformation to ethylene activity. One of selected endophytic bacteria was identified as Bacillus megatherium using Biolog Microlog Bacterial Identification System.
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