Impact of macro-elements on oxalic acid biosynthesis process by Aspergillus niger with glycerol
2011
Walaszczyk, E. | Podgórski, W. | Marzec, D.,UE Wro-Uniwersytet Ekonomiczny, Uniwersytet Ekonomiczny, Wroclaw (Poland). Instytut Chemii i Technologii Zywnosci
The objective of this paper was to study the possibility of utilizing glycerol as a source of carbon and energy in the oxalic acid biosynthesis process by Aspergillus niger W78C in the shaking flasks-based cultures, as well as to determine the optimum concentration of macro-elements in the medium applied in the process. The best bioprocess results, more than 43 g minus cubic dm of oxalic acid, were obtained in the variant of the medium containing: 1,30 g minus cubic dm NH4NO3, and 0,3 g minus cubic dm of KH2PO4 and MgSO4x7H2O. The lack of phosphorus in the medium was a critical factor as regards the growth of biomass, and this fact significantly limited the production of oxalic acid. Nitrogen was found to be, also, an important component of the medium. The culture developed in the variant with a too low concentration level of this element was characterized by a low growth level of biomass and by low values of the product concentration. The preference of magnesium salt was not critical for the process to run
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