Radiosensitivity of sunflower inbred lines to mutagenesis
2012
Cvejić S., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Afza, R., Joint FAO/IAEA Agriculture and Biotechnology Laboratory, Seibersdorf (Austria) | Jocić, S., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Prodanović, S., Faculty of Agriculture, Belgrade - Zemun (Serbia) | Miklič, V., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Škorić, D., Serbian Academy of Sciences and Arts (SASA), Novi Sad (Serbia). Branch in Novi Sad | Dragin, S., Ministry of Agriculture, Belgrade (Serbia)
For much of the past century, mutagenesis has gained popularity in plant genetics research as a means of inducing novel genetic variation. Induced mutations have been applied for the past 40 years to produce mutant cultivars in sunflower by changing plant characteristics that significantly increase plant yield and quality. The present study is focused on generating baseline data to elucidate the role of genotypic differences in the response of sunflower to induced mutagenesis with the aim of expanding the applicability of the use of induced mutant stocks in the genetic improvement of the crop and in its functional genomics. The strategy adopted was to estimate the optimal treatment conditions (doses of mutagens) through relating the extent of damage in seedling progeny to the exposure levels of the initiating propagates to mutagens. Seeds of fifteen elite sunflower genotypes of commonly used as breeding stocks and grown on commercial scales were treated with a range of mutagens: gamma rays (G rays); fast neutrons and with ethyl-methane-sulphonate (EMS) at different treatment doses. The three mutagenic agents affected seedling height, reducing it with increasing dosage. Based on the mutagen damage on seedling height, the 50% and 30% damage indices (D50 and D30, respectively) were estimated for the 15 sunflower genotypes for the three mutagens. The D50 (D30) values for the sunflower lines ranged from 120 to 325 Gy (5 to 207 Gy) for gamma irradiation; 9 to 21 Gy (0.1 to 10 Gy) for fast neutrons and 0.69 to 1.55% (0.01 to 0.68%) concentration of EMS.
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