Isolation and molecular characterization of toxoplasma gondii from chickens in china
Zhao Guangwei, Nanjing Agricultural University, Nanjing (China), College of Veterinary Medicine | Fan Honggang, Nanjing Agricultural University, Nanjing (China), College of Veterinary Medicine | Xie Qing, Nanjing Agricultural University, Nanjing (China), College of Veterinary Medicine
One strain of Toxoplasma gondii was successfully isolated from chickens in China by bioassay in mice. Antibodies and circulating antigens of T. gondii were assayed by the ELISA kits in 100 free range chickens from a rural area surrounding Funing, China. Fifty-three chickens were antibody-positive and 21 chickens were antigen positive. Hearts, brains, spleens, lungs, livers, and kidneys of 21 antibody or antigen-positive chickens were bioassayed in mice. One strain of T. gondii was isolated from 1 of 21 (4.76%) chickens. The isolated T. gondii killed all of the inoculated mice. Genotyping of this isolate using polymorphisms at the loci 5_SAG2, 3_SAG2, SAG3, cB21-4, L358, BTUB, and GRA6 revealed that it was Type I. These indicated that it was virulent for mice. This is the first report of isolation of T. gondii from chickens in China.
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