Genetic diversity of Czech apple cultivars inferred from microsatellite markers analysis
Patzak, J., Chmelarsky Inst., Zatec (Czech Republic) | Paprstein, F., Vyzkumny a Slechtitelsky Ustav Ovocnarsky, Holovousy (Czech Republic) | Henychova, A., Chmelarsky Inst., Zatec (Czech Republic) | Sedlak, J., Vyzkumny a Slechtitelsky Ustav Ovocnarsky, Holovousy (Czech Republic)
Genetic diversity and genetic relationships of Czech apple cultivars were evaluated. Trees of 33 Czech apple cultivars and 97 reference foreign cultivars were analysed using the set of 10 simple sequence repeat primer pairs. In total, 89 polymorphic alleles were amplified, while the number of alleles per locus ranged from 4 to 14. The SSR dendrogram, based on the Jaccard's similarity coefficient, divided apple cultivars into three major groups: Cox's Orange Pippin, McIntosh and Golden Delicious ancestries. The clustering highly depended on pedigree and origin of apple cultivars. Spontaneous mutated cultivars were identical with their progenitors. We proved that microsatellite markers were useful in evaluating genetic resources, collection management and cultivar identification.
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