Effect of supplementation of various selenium forms and doses on selected parameters of ruminal fluid and blood in sheep
Panev. A., University of Veterinary and Pharmaceutical Sciences, Brno (Czech Republic). Ruminant and Swine Clinic | Hauptmanova, K., University of Veterinary and Pharmaceutical Sciences, Brno (Czech Republic). Ruminant and Swine Clinic | Pavlata, L., University of Veterinary and Pharmaceutical Sciences, Brno (Czech Republic). Ruminant and Swine Clinic | Pechova, A., University of Veterinary and Pharmaceutical Sciences, Brno (Czech Republic). Ruminant and Swine Clinic | Filipek, J., University of Veterinary and Pharmaceutical Sciences, Brno (Czech Republic). Ruminant and Swine Clinic | Dvorak, R., University of Veterinary and Pharmaceutical Sciences, Brno (Czech Republic). Ruminant and Swine Clinic
Effect of various doses and forms of selenium (organically bound vs. inorganic) on selected parameters of ruminal fluid and blood in sheep was evaluated. The trial was performed with 15 sheep divided into two groups. Animals from group A (n = 8) received a feeding mixture with selenomethionine, while sheep from group B (n = 7) were fed a mixture with sodium selenite. During the first 14 days, animals from both groups were fed a mixture with optimum dose of Se (1 mg Se/kg dry matter). For another 28 days, all experimental animals received a Se-deficient mixture (0.1 mg Se/kg dry matter), whereas in the last 21 days of the experiment, animals were fed a high-Se diet (5.0 mg Se/kg dry matter). Throughout the trial, 4 samples of blood and ruminal fluid were taken from each animal. The samples of ruminal fluid were analyzed to determine the concentration of Se and identify other parameters of ruminal fermentations. Selenium levels were also determined in ruminal biomass. In blood, Se concentration, glutathione peroxidase (GSH-Px) activity, and other selected biochemical parameters were measured. The results of the present study demonstrate that the actual intake of both organic and inorganic Se is reflected in Se concentration in ruminal fluid and ruminal biomass and, similarly, in Se content and GSH-Px activity in blood. The form of supplemented Se did not have a significant effect on ruminal fermentation parameters in dependence on different doses of Se contained in feeding rations (except for the negative effect of a sudden start of feeding high levels of organically bound Se on infusoria count, which was accompanied by the increase of GMT, LDH, and AST enzymes activity in ruminal fluid). The results also suggest possible negative interaction between the intake of organically bound selenium and the concentration of copper in blood of sheep.
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