Non-symbiotic hemoglobin and nitrate reductase constitute an alternative to fermentation in waterlogging tolerance of mung bean (Vigna radiata (L.) Wilczek)
Sairam, R.K. | Chinnusamy, V. | Arora, A. | Bhattacharya, P. | Joshi, R. | Trivedi, S.
The objective of this study was to examine the role of nitrate reductase, nitric oxide and non-symbiotic hemoglobin in imparting waterlogging tolerance in mung bean genotypes. Experiment was conducted with five cultivated mung bean [Vigna radiata (L.) Wilczek] genotypes viz., T 44, MH 96-1 (relatively tolerant), and Pusa Baisakhi, MH lK-24 and PS 10 (susceptible) under pot-culture condition. Waterlogging induced reduction in relative water content, chlorophyll content and membrane stability index was comparatively less in tolerant genotypes T 44, MH 96-1 than in Pusa Baisakhi, MH lK-24 and PS 10. The nitric oxide (NO) production activity increased up to 6 days of waterlogging treatment in all the genotypes, however T 44, MH 96-1 maintained more than double NO content than Pusa Baisakhi, MH lK-24 and PS 10. Increase in nitrate reductase (NR) activity under waterlogging was observed up to 6-days of treatment in all the genotypes, and T 44, MH 96-1 maintained significantly higher NR activity than Pusa Baisakhi, MH lK-24 and PS 10. Non-symbiotic hemoglobin (NSHb) and cNR mRNA expressions were studied only in the roots of control and waterlogging treated plants of T 44, MH 96-1, and PS 10. Waterlogging induced increase in expression was observed only in tolerant genotypes T 44 and MH 96-1, while little expression was observed in PS 10. In this study we have reported the involvement of non-symbiotic hemoglobin-nitric oxide homeostasis in the waterlogging tolerance of mung bean.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Indian Council of Agricultural Research