Physiological and Proteomic Responses of Barley Seedlings to Salt Stress
2011
Kim, D.W., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Yun, S.K., Chonbuk National University, Jeonju, Republic of Korea | Park, H.H., National Institute of Crop Science, RDA, Iksan, Republic of Korea | Hwang, J.J., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Han, O.K., National Institute of Crop Science, RDA, Iksan, Republic of Korea | Park, T.I., National Institute of Crop Science, RDA, Iksan, Republic of Korea | Jung, G.H., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Lee, J.E., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Kim, S.L., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Chung, Y.H., Korea Basic Science Institute, Daejeon, Republic of Korea
This study was conducted to obtain basic information on physiological and proteomic responses of barley seedlings to salt stress. Shoot dry weight decreased significantly as the level of soil salinity increased. Salt stress-induced decrease of relative shoot dry weight was lower in cv. "Sanglok" than in cv. "Sunwoo". Under the salt stress, SPAD value decreased, and the value was higher in cv. "Sanglok" than in cv. "Sunwoo". Sodium ion content in the leaves increased as NaCl concentration increased, and the content was higher in cv. "Sunwoo" than in cv. "Sanglok". The K+/Na+ratio was higher in cv. "Sanglok" than in cv. "Sunwoo". Salt stress-induced alterations in protein expression of the leaves were detected by two dimensional electrophoresis, and 47 protein spots showing altered expression were selected. Among the selected protein spots, 17 protein spots were up-regulated and 28 spots down-regulated in cv. "Sanglok". In cv. "Sunwoo", 14 protein spots were up-regulated and 27 spots down-regulated. Out of 47 deferentially expressed protein spots, 18 protein spots were identified using mass spectrometry and NCBI protein database. Among the identified proteins, ten proteins are known to be involved in various stress responses, but the others are not directly involved in stress responses.
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