Molecular characterization of Cymbidium mosaic virus (Cym MV) infecting orchids in Java, Indonesia
2011
Lakani, I. | Suastika, G. | Damayanti, T.A. | Mattjik, N., Tadulako Univ., JI Sam Ratulangi No. 70a Palu (Indonesia)
Cymbidium mosaic virus (Cym MV) is an economically important virus infecting orchids in the world. The typical symptoms such mosaic and necrotic on the leaves were found infecting Cymbidium, Phalaenopsis, Dendrobium, and Oncydium in Indonesia. The disease is distributed in several areas in Java and were suspected spread via vegetative materials. The objective of the research was to identify the causal of mosaic disease infecting orchids. Symptomatic samples were collected from several areas such as from Surabaya and Malang (East Java), Magelang (Central Java), Jakarta, and Bandung and Bogor (West Java). Serological test of all samples were positive against Cym MV antisera. The virus could infect several indicator plants such as Datura stramonium, Chenopodium amaranticolor, C. quinoa, Nicotiana benthamiana, N.glutinosa and Cassia occidentalis. Further, reverse transcription polymerase chain reaction (RT-PCR) of total RNA extracted from symptomatic orchids were carried out by using a pair of primer specific to the coat protein of Cym MV. The 650 bp DNA fragment was successfully amplified,confirming the presence of Cym MV in those samples. Direct sequencing of the CP gene showed that the homology among those isolates ranged from 90.1-97.7%, while to those of isolates from other countries range from 92.8 to 98.3%. Phylogenetic analysis of the CP amino acid and sequences revealed that those six Indonesian isolates were clustered in the same group with those isolated from Japan, India, China and South Korea. This is the first report of Cym MV infecting orchids in Java, Indonesia.
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