Regulatory expression and cellular localization of doublecortin in the rat retina following ischemia-reperfusion injury
2011
Gwon, J.S., The Catholic University of Korea, Seoul, Republic of Korea | Chun, M.H., The Catholic University of Korea, Seoul, Republic of Korea | Kang, W.S., The Catholic University of Korea, Seoul, Republic of Korea
Doublecortin (DCX) is microtubule-associated protein and is required for neuronal migration, differentiation and plasticity. In the retina, it is highly expressed between embryonic day 18 (E18) and E20, and is poorly expressed postnatally. In this study, we investigated the expression and cellular localization of DCX in the rat retina following ischemia induced by transiently increasing the intraocular pressure. While DCX immunoreactivity in control retinas was restricted to the outer border of the inner nuclear layer, it appeared in horizontal cell somata and processes in affected retinas. Quantitative evaluation by immunoblotting confirmed that DCX expression continuously increased after ischemia-reperfusion and showed 370% of control protein levels at 4 weeks after ischemic insult. These results suggest that the DCX in horizontal cells might play a role in neurite remodeling or modulating other neurons in ischemic rat retinas.
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