Analysis of Eating Quality in Recombinant Inbred Lines and Selection of Elite Line with Low Glutelin Content in Rice
2012
Lee, J.Y., National Institute of Crop Science, RDA, Miryang, Republic of Korea | Lee, J.H., National Institute of Crop Science, RDA, Miryang, Republic of Korea | Cho, J.H., National Institute of Crop Science, RDA, Miryang, Republic of Korea | Kim, S.Y., National Institute of Crop Science, RDA, Miryang, Republic of Korea | Kim, C.S., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Sohn, Y.B., National Institute of Crop Science, RDA, Miryang, Republic of Korea | Yeo, U.S., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Song, Y.C., National Institute of Crop Science, RDA, Miryang, Republic of Korea | Lee, C.W., National Institute of Crop Science, RDA, Miryang, Republic of Korea | Nam, M.H., National Institute of Crop Science, RDA, Miryang, Republic of Korea
In chronic renal failure, to reduce the burden on the kidneys, it is necessary to provide patients with therapy based on a low-protein diet. This study was conducted to improve eating quality for developing low easy to-digest protein rice variety with Lgc1 gene. Two hundred twenty six recombinant inbred lines (RILs) were used, which were derived from the cross between Chugoku173, a low glutelin rice variety developed in Japan, and Junambyeo, a high quality rice variety in Korea. We investigated glutelin phenotype using SDS PAGE and its genotype by functional DNA marker. As results of them were consistent, this DNA marker is expected to use of MAS for the Lgc1- mediated low glutelin variety. The eating quality of rice measured by Toyo meter (GMT) showed significantly negative correlation with amylose content, but there was no significance with protein content in this population. Dividing the RILs into normal amylose group (app. 19%) and low amylose group (app. 13%), the eating quality estimated by GMT was generally lower in low glutelin lines, but that of low amylose group was higher than that of normal amylose group. On the basis of several results, it is supposed that low amlyose gene can be used to breed the low glutelin rice variety with high eating quality. Through the analysis of agronomic traits related to eating quality, Milyang262, low glutelin and amylose rice variety, were developed.
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