PCR-based detection of aphids in the gut contents of arthropod predators
2013
Aslam, M. (Government of Punjab, Bahawalpur (Pakistan). Dept. of Fisheries) | Ruby, T. (The Islamia Univ. of Bahawalpur (Pakistan). Dept. of Life Sciences) | Ghaffar, A. (The Islamia Univ. of Bahawalpur (Pakistan). Dept. of Life Sciences) | Farooq, Z. (Government of Punjab, Vehari (Pakistan). Dept. of Wildlife) | Hussain, T. (The Islamia Univ. of Bahawalpur (Pakistan). Coll. of Agriculture and Environmental Sciences) | Rafay, M.
Fodders (maize, sorghum, chari, shaftal and alfalfa), wheat and mustard crops were sampled round the year in the cropland of Punjab. The most abundant pest species of the cropland were aphids namely Aphis maidis, Schizaphis graminum, and Diuraphis noxia, which were subjected to gut analysis. Species-specific primers of these aphid species were applied on the selected predators. Coccinella septumpuctata and Oxyopes javanus were positive for the consumption of A. maidis (corn aphid), Hippodemia convergens for S. graminum (wheat aphid), while Neoscona theisi was positive for Diuraphis noxia (wheat aphid). Such findings seemed to be helpful for implementation of species specific biological control against a specific pest in our agro-ecosystems.
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