The enantiomer distribution of major chiral volatile organic compounds in Slovakian monofloral honeys
2012
Pažitná, A., Slovak University of Technology, Bratislava (Slovak Republic) | Janáčová, A., Slovak University of Technology, Bratislava (Slovak Republic) | Špánik, I., Slovak University of Technology, Bratislava (Slovak Republic)
In this work, the distribution of enantiomers of selected chiral volatile organic compounds in 45 monofloral honey samples was studied by gas chromatography (GC). The volatile organic compounds were extracted from Slovakian rapeseed, acacia, sunflower basswood and raspberry honeys by solid phase microextraction followed by gas chromatography – mass spectrometric (GC-MS) analysis. The chiral compounds present at higher contents were selected from 230 organic compounds found in studied samples for determination of their isomeric ratios. It was found that one-dimensional GC with chiral stationary phases provided excellent enantiomer separations; however, the resolved enantiomers often co-eluted with other non-chiral or already separated enantiomers of other organic compounds. Thus, two-dimensional GC with two independent thermostats and proper column setup was required to determine correct isomeric ratios. Finally, the isomeric ratios of linalool, cis- and trans-furanoid linalool oxides, hotrienol and four isomers of lilac aldehydes were determined. It was found that distribution of enantiomers in honey samples partially depended on their botanical origin. The differences in ratios of lilac aldehyde isomer B and hotrienol were observed for acacia honey that allowed us to distinguish this type of honey from others. Similarly, a different isomeric ratio of trans-furanoid linalool oxide was found for sunflower honeys.
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