Effect of Bacillus spp. on inhibition of pathogenic bacteria Aeromonas hydrophila and Streptococcus agalactiae in nile tilapia (Oreochromis niloticus)
2011
Patarida Podok(Kasetsart University, Bangkok (Thailand). Faculty of Fisheries. Aquaculture Business Research Center) | Chalor Limsuwan(Kasetsart University, Bangkok (Thailand). Faculty of Fisheries. Department of Fishery Biology. Aquaculture Business Research Center) | Watchariya Purivirojkul(Kasetsart University, Bangkok (Thailand). Faculty of Science. Department of Zoology) | Niti Chuchird(Kasetsart University, Bangkok (Thailand). Faculty of Fisheries. Department of Fishery Biology. Aquaculture Business Research Center)
Experiment has been carried out to determine the efficacy of three species of Bacillus (B. licheniformis, B. pumilus and B. subtilis) on inhibition of pathogenic bacteria (Aeromonas hydrophila ABRCA1 and Streptococcus agalactiae ABRCS1) from Nile tilapia (Oreochromis niloticus). The bacterial antagonistic activity by cross streak method showed that B. licheniformis could inhibit A. hydrophila ABRCA1 after 48 hours of incubation at 37 deg C. B. licheniformis, B. pumilus and B. subtilis could colonize over S. agalactiae ABRCS1 after 48 hours of incubation at 37 deg C. Three species of Bacillus spp. which shown colonization ability were tested for ability to decrease pathogenic bacteria S. agalactiae ABRCS1 by broth co-culture method. B. licheniformis, B. pumilus, B. subtilis and S. agalactiae ABRCS1 were cultured with the initial concentration about 105 CFU/ml. After 48 hrs of culture, S. agalactiae ABRCS1 when co-cultured in nutrient broth (NB) with B. licheniformis, B. pumilus and B. subtilis respectively were decreased by 46.84 percent, 53.57 percent and 44.28 percent, respectively. The results from this study can suggest that these Bacillus spp. strains can use for decrease pathogenic bacteria in Oreochromis niloticus culture.
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Эту запись предоставил Kasetsart University