Methylglyoxal with glycine or succinate enhances differentiation and shoot morphogenesis in Nicotiana tabacum callus
Ray, A., Indian Association for the Cultivation of Science, Kolkata (India). Dept. of Biological Chemistry | Ray, S., 13 Regent Estate, Kolkata (India) | Mukhopadhyay, S., University of Calcutta, Kolkata (India). Dept. of Botany | Ray, M., Bose Institute, Kolkata (India). Dept. of Molecular Medicine
The aim of this study was evaluate the influence of methylglyoxal (MG) on organogenesis nad regeneration of tobacco (Nicotiana tabacum) plants from callus in media containing glycine or succinate. The best improvement in shoot proliferation and shoot lenght was obtained in the medium supplemented with 0.1 mM MG and 0.5 mM glycine or 0.5 mM succinate. The histological studies showed vigorous development of corm like structures and shoot organogenesis from callus tissues cultured in MG supplemented media. Biochemical studies also revealed higher content of delta-aminolaevulinic acid (a precursor of chlorophyll) and of chlorophyll.
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