Sanitation of currant cultivars
2011
Sedlak, J., Vyzkumny a Slechtitelsky Ustav Ovocnarsky, Holovousy (Czech Republic) | Paprstein, F., Vyzkumny a Slechtitelsky Ustav Ovocnarsky, Holovousy (Czech Republic) | Spak, J., Biology Centre of the Academy of Sciences of the Czech Republic, Ceske Budejovice (Czech Republic) | Pribylova, J., Biology Centre of the Academy of Sciences of the Czech Republic, Ceske Budejovice (Czech Republic) | Svobodova, L., Vysoka Skola Chemicko-technologicka, Prague (Czech Republic). Ustav Chemie a Analyzy Potravin
The aim of this work was to determine the health status of primary resources of red currant cultivars Detvan, Rubigo, Vitan and white currant cultivar Orion and research of thermotherapy in 39 deg C as a method of sanitation from viral diseases. BRV (Black currant reversion virus) in primary resources of Detvan, Vitan, Orion and GVBaV (Gooseberry vein banding associated virus) in primary resources of Rubigo, Vitan, Orion were detected by PCR. The detection of GVBaV is the first experimental proof of this virus on the territory of the Czech Republic. Phytoplasmas were not present in PCR tested plants. SLRSV (Strawberry latent ringspot virus) was detected by ELISA testing in the bush of red currant cultivar Detvan. Based on the preliminary results of testing, one clone of red currant cultivar Rubigo was sanitated from GVBaV, one clone of red currant cultivar Detvan from mixed infection of two viruses SLRSV and BRV and two clones of white currant cultivar Orion from mixed infection of two viruses BRV and GVBaV. Both clones of red currant cultivar Vitan remained infected with one of two viruses (BRV or GVBaV), which were detected in initial bush before the beginning of thermotherapy. Sanitation and testing of currant cultivars used in the project will be carried out in the following years.
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