Botanical studies on the micropropagation of two dried cv. S, from date palm, phoenix dactylifera, L. using tissue culture technique 2-carbohydrates fractions and acclimatization
2009
Helaly, M.N. | Hegazy, A.E.A. | El-Hosieny, H.A.M.
Bartamuda cv has higher level of all carbohydrates fractions studied and least values of total proteins, amino acids and proline concentrations compared with Sakkoty cv overall the developmental stages. The possibility that Sakkoty cv can form specific protein types for adaptation was suggested. The concentrations of reducing sugars, non reducing sugars and total sugars were increased whereas total protein and amino acids were decreased with subculturing. The lowest significant values of carbohydrate fractions and the highest values of total protein , amino acids and praline were noticed at the embryogenic stags. Polysaccharides and insoluble sugars were decreased gradually in Bartamyda cv. whereas in Sakkoty cv, up to the embryonic callus and thereafter increased The interaction treatments showed that the highest significant value of carbohydrates fractions were recorded with Bartamuda cv at initial exp1ant and callus stages in addition to Sakkoty cv at only the callus stage. The lowest significant values were found with Sakkoty cv at the embryogenic stage. At plantlet stage, a slight increases in all carbohydrates formations concentrations were noticed, but still less than the corresponding calculated values recorded at stages of the initial shoot tip explants and callus initiations.Total protein and amino acids were decreased at callus stage gradually up to the embryogenic callus and thereafter increased at plantlet stage to reached vales more than the corresponding initial explant. These results are true in the two cv (s) studied.
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