Characterization of a new, nonpathogenic mutant of Botrytis cinerea with impaired plant colonization capacity
2006
Kunz , Caroline (INRA , Paris (France). UMR 0217 Interactions Plantes Pathogènes) | Vandelle , Elodie (INRA Institut National de la Recherche AgronomiqueCentre National de la Recherche Scientifique, DijonDijon(France). UMR1088 Plante-Microbe-Environnement, Laboratoire de Signalisation cellulaire et moléculaire dans les réactions de défenseUMR5184 Laboratoire de Signalisation cellulaire et moléculaire dans les réactions de défense, Université de Bourgogne) | Rolland , Stéphane (Centre National de la Recherche Scientifique, Villeurbanne(France). UMR5122 CNRS/INSA/Université Lyon 1, Laboratoire de Biologie Cellulaire Fongique) | Poinssot , Benoît (INRA , Dijon (France). UMR 1088 Plante - Microbe - Environnement) | Bruel , Christophe (Centre National de la Recherche Scientifique, Villeurbanne(France). UMR5122 CNRS/INSA/Université Lyon 1, Laboratoire de Biologie Cellulaire Fongique) | Cimerman , Agnes (INRA , Paris (France). UMR 0217 Interactions Plantes Pathogènes) | Zotti , Corine (Université Pierre et Marie Curie, Paris(France). Laboratoire de Biochimie et Pathologie Végétales) | Moreau , Elisabeth (Université Pierre et Marie Curie, Paris(France). Laboratoire de Biochimie et Pathologie Végétales) | Vedel , Régine (INRA (France). UMR 0217 UMR INRA / INA PG / Univ. Paris 6 : Pathologie Végétale) | Pugin , Alain (INRA , Dijon (France). UMR 1088 Plante - Microbe - Environnement) | Boccara , Martine (INRA (France). UMR 0217 UMR INRA / INA PG / Univ. Paris 6 : Pathologie Végétale)
Botrytis cinerea is a necrotrophic pathogen that attacks more than 200 plant species.Here, the nonpathogenic mutant A336, obtained via insertional mutagenesis, was characterized.Mutant A336 was nonpathogenic on leaves and fruits, on intact and wounded tissue, while still able to penetrate the host plant. It grew normally in vitro on rich media but its conidiation pattern was altered. The mutant did not produce oxalic acid and exhibited a modified regulation of the production of some secreted proteins (acid protease 1 and endopolygalacturonase 1). Culture filtrates of the mutant triggered an important oxidative burst in grapevine ( Vitis vinifera ) suspension cells, and the mutant–plant interaction resulted in the formation of hypersensitive response-like necrosis. Genetic segregation analyses revealed that the pathogenicity phenotype was linked to a single locus, but showed that the mutated gene was not tagged by the plasmid pAN7-1.Mutant A336 is the first oxalate-deficient mutant to be described in B. cinerea and it differs from all the nonpathogenic B. cinerea mutants described to date.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Institut national de la recherche agronomique
