Sultr4;1 mutant seeds have an enhanced sulphate content and modified proteome suggesting metabolic adaptations to altered sulphate compartmentalization
Zuber , Helene (INRA , Dijon (France). UMR 0102 Unité de Recherche Génétique et Ecophysiologie des Légumineuses) | Davidian , Jean-Claude (INRA , Montpellier (France). UMR 0386 Biochimie et Physiologie Moléculaire des Plantes) | Wirtz , Markus (Heidelberg University, Heidelberg(Allemagne). Heidelberg Institute of Plant Sciences) | Hell , Rüdiger (Heidelberg University, Heidelberg(Allemagne). Heidelberg Institute of Plant Sciences) | Belghazi , Maya (Centre National de la Recherche Scientifique, Marseille(France). Centre d’Analyse Protéomique de Marseille, CAPM, IFR Jean Roche) | Thompson , Richard (INRA , Dijon (France). UMR 0102 Unité de Recherche Génétique et Ecophysiologie des Légumineuses) | Gallardo , Karine (INRA , Dijon (France). UMR 0102 Unité de Recherche Génétique et Ecophysiologie des Légumineuses)
Background: Sulphur is an essential macronutrient needed for the synthesis of many cellular components. Sulphur containing amino acids and stress response-related compounds, such as glutathione, are derived from reduction of root-absorbed sulphate. Sulphate distribution in cell compartments necessitates specific transport systems. The lowaffinity sulphate transporters SULTR4;1 and SULTR4;2 have been localized to the vacuolar membrane, where they may facilitate sulphate efflux from the vacuole. Results: In the present study, we demonstrated that the Sultr4;1 gene is expressed in developing Arabidopsis seeds to a level over 10-fold higher than the Sultr4;2 gene. A characterization of dry mature seeds from a Sultr4;1 T-DNA mutant revealed a higher sulphate content, implying a function for this transporter in developing seeds. A fine dissection of the Sultr4;1 seed proteome identified 29 spots whose abundance varied compared to wild-type. Specific metabolic features characteristic of an adaptive response were revealed, such as an up-accumulation of various proteins involved in sugar metabolism and in detoxification processes. Conclusions: This study revealed a role for SULTR4;1 in determining sulphate content of mature Arabidopsis seeds. Moreover, the adaptive response of sultr4;1 mutant seeds as revealed by proteomics suggests a function of SULTR4;1 in redox homeostasis, a mechanism that has to be tightly controlled during development of orthodox seeds.
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