Ozone effects on stomatal responses to environmental parameters (blue light, red light, CO2 and VPD) in three poplar genotypes and the study of gene expression on guard cells
Dumont , Jennifer (INRA , Champenoux (France). UMR 1137 Ecologie et Ecophysiologie Forestières) | Spicher , Fabien (INRA , Champenoux (France). UMR 1137 Ecologie et Ecophysiologie Forestières) | Cohen , David (INRA , Champenoux (France). UMR 1137 Ecologie et Ecophysiologie Forestières) | Ningre , Nathalie (INRA , Champenoux (France). UMR 1137 Ecologie et Ecophysiologie Forestières) | Gérard , Joëlle (INRA , Champenoux (France). UMR 1137 Ecologie et Ecophysiologie Forestières) | Montpied , Pierre (INRA , Champenoux (France). UMR 1137 Ecologie et Ecophysiologie Forestières) | Jolivet , Yves (INRA , Champenoux (France). UMR 1137 Ecologie et Ecophysiologie Forestières) | Dizengremel , Pierre (INRA , Champenoux (France). UMR 1137 Ecologie et Ecophysiologie Forestières) | Le Thiec , Didier (INRA , Champenoux (France). UMR 1137 Ecologie et Ecophysiologie Forestières)
Tropospheric ozone acts as a phytotoxin which produces an oxidative stress in plants. Two ways of defense are used, either by preventing ozone input through the regulation of stomatal conductance, or by detoxifying ozone and ROS in cells. It is known that stomatal movements are altered by ozone. We performed fumigation experiment on three euramerican poplar genotypes (Populus deltoides x Populus Nigra : ‘Carpaccio’, ‘Cima’ and ‘Robusta’), cultivated in pots in phytotronic chambers submitted to 120 ppb ozone or filtered air. We explored the effects of ozone on stomatal responses to four environmental parameters (blue light, red light, CO2 and VPD) using Licor6400. We also find out using a porometer that the decrease of stomatal conductance due to ozone is earlier on the adaxial face than on abaxial face. Finally, to better understand these impacts, we studied ultrastructure of guard cells by TEM, stomatal density and size of stomata by SEM, and we performed X-ray microanalysis of guard cells mineral content. These approaches are coupled with the study on microdissected stomata of expression of genes involved in regulation of stomatal movements
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