A high-density genetic map of the Medicago truncatula major freezing tolerance QTL on chromosome 6 reveals colinearity with a QTL related to freezing damage on Pisum sativum linkage group VI
2013
Tayeh , Nadim (INRA , Dijon (France). UMR 1347 Agroécologie) | Bahrman , Nasser (INRA , Peronne (France). UMR 1281 Stress Abiotiques et Différenciation des Végétaux Cultivés) | Devaux , Rosemonde (INRA , Peronne (France). UE 0972 Unité expérimentale de Lille) | Bluteau , Aurélie (Institut National de la Recherche Agronomique, Estrées-Mons(France). UMR 1281 SADV) | Prosperi , Jean-Marie (INRA , Montpellier (France). UMR 1334 Amélioration Génétique et Adaptation des Plantes méditerranéennes et Tropicales) | Delbreil , Bruno(auteur de correspondance) (INRA , Peronne (France). UMR 1281 Stress Abiotiques et Différenciation des Végétaux Cultivés) | Lejeune-Henaut , Isabelle (INRA , Peronne (France). UMR 1281 Stress Abiotiques et Différenciation des Végétaux Cultivés)
Freezing is one of the most serious abiotic stress factors that affect cool-season legumes. It limits species geographic distribution and causes severe yield losses. Improving tolerance to freezing has long been a main concern for legume breeders. Medicago truncatula Gaertn. has been selected as a model species for legume biology. Various studies have shown significant macrosynteny between M. truncatula and agronomically important crop legumes. A major freezing tolerance quantitative trait locus (QTL), herein referred to as Mt-FTQTL6, was previously identified on M. truncatula chromosome 6. The physical location of this QTL was determined in this study and its corresponding chromosomal interval was enriched with additional markers. Markers were first developed using the draft sequence of M. truncatula euchromatin (release versions Mt3.0 and Mt3.5). Because Mt-FTQTL6 was found to coincide with an assembly gap, the Glycine max (L.) Merr. genome sequence was also used to generate markers. Five Mt-FTQTL6-linked markers were found to be common to a region on Pisum sativum L. linkage group VI harboring a QTL for freezing damage. A subset of markers was tested for transferability across 11 additional legume species. This study lays the groundwork for identifying the molecular basis of Mt-FTQTL6. Cross-legume markers will be useful in future efforts aiming to investigate the conservation of Mt-FTQTL6 in cool-season legumes and subsequently the existence of common mechanisms for response to freezing between M. truncatula and crop legumes.
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