Genetic purity testing of rice hybrid seeds using microsatellite markers
2012
Mirmohammadi-Maibody, S. A.M., Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology | Hashemi-Petroudi, S.H., Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology | Arzani, A., Rice and amp; Citrus Research Institute, Sari University of Agricultural Sciences and Natural Resources | Nematzadeh, Gh., Rice and amp; Citrus Research Institute, Sari University of Agricultural Sciences and Natural Resources
Identification and determination of genetic purity of rice hybrid varieties for seed certification and control is an essential issue regarding to apply hybrid rice technology in Iran. Therefore, usefulness of microsatellite markers (SSR) for fingerprinting and genetic purity testing were investigated. Eight F1 rice hybrids and their parental lines were evaluated using 14 microsatellite markers in which 10 markers had reproducible polymorphic bands among genotypes. Totally 27 bands (alleles) produced, average number of band per primers and per genotypes were 1.92 and 1.68, respectively. Genetic purity was tested in respect of microsatellite fingerprint and polymorphic primers which detected off types seeds among F1 hybrids. Extra band(s) was also observed when the F1's SSR products were analyzed. Null alleles were appeared in three restorer lines for RM264 microsatellite locus. Cluster analysis based on shared allelic similarity coefficient, using UPGMA algorithm, grouped the 16 rice genotypes into three main clusters, each containing 43.75%, 43.75% and 12.5% of genotypes. Overall, these results indicated there are reliable tools of microsatellite markers in DNA profiling and seed purity testing in rice varieties, especially hybrids.
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