Chemical and electrical activation of swamp buffalo (Bubalus bubalis) oocytes for the production of parthenogenetic embryos in-vitro
2011
Atabay, E.C. | Atabay, E.P. | Mamuad, F.V. | Cruz, L.C.
The development of efficient procedure for the activation of in vitro matured swamp buffalo oocytes is important towards efficient utilization of the limited buffalo oocytes for cloning. The present study was conducted to compare the effects of chemical or electrical stimulus on the induction of activation of swamp buffalo oocytes, and to investigate the effects of aging on post-parthenogenetic development. Bubaline oocytes matured in vitro for 24 or 40 h were activated with ethanol or electrical stimulus with or without cycloheximide (CHX) treatment. Activation and cleavage rates tended to be higher at 40 h post maturation than those activated at 24 h. However, a significant decrease was observed when activated oocytes were not exposed to CHX, regardless of the activation treatment (P0.01). Significant differences were observed in the development of parthenotes to morula and blastocyst stage when activation treatment was applied at 24 h compared to those activated at 40 h post maturation, regardless of the activation treatment. The present study demonstrated the possibility of in-vitro matured swamp buffalo methapase 2 oocytes at 24 h by electric stimulation or with ethanol followed by cycloheximide treatment without deleterious effect on subsequent development of parthenotes.
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