Diversity analysis of sweet potato (Ipomoea batatas (L.) Lam) germplasm collection of the National Plant Genetic Resources Laboratory (NPGRL) using microsatellite markers
2013
Rama, R.A.B. | Pabro, L.J.A. | Lalusin, A.G.
Microsatellite markers or simple sequence repeat (SSR) were used to determine the genetic diversity of sixty four sweet potato accessions of National Plant Genetic Resources Laboratory, Institute of Plant Breeding in the University of the Philippines, Los Baños. Ten SSR markers based on three different crops (Ipomoea batatas-based, Musa-based and Allium-based markers) were used. Eight of these ten primer pairs amplified a range of 2 to 8 polymorphic fragments per primer. Resulting unique banding patterns for the 10 SSR markers ranged from 1 to 21. The resolving power of the SSR markers used, measured as polymorphism information content (PIC), ranged from 41.14% to 83.90%, which indicates high genetic diversity. Highest PIC was observed in marker mMaC1R1-69, as well as the most number of banding patterns. Profiles of the markers used were subjected to cluster analysis using Jaccard's coefficient at 0.51 which showed high genetic diversity among accessions. The resulting dendrogram generated five distinct clusters. Grouping based on geographic location was observed. Knowledge about sweet potato germplasm diversity could be an important aide in crop improvement strategies.
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