Novel plant acid phosphatases from the orphan crop Lagenaria siceraria (Molina) Standl. for phosphate ester sysnthesis
2012
Koffi, D.M. | Bedikou, M.E. | Niamke, S.L., Filiere Biochimie-Microbiologie de l'Unite de Formation et de Recherche Biosciences de l'Universite de Cocody, 22 BP 582 Abidjan (Cote de'Ivoire). Laborntoire de Biotechnologies | Faulet, B.M. | Gonnety, J.J. | Kouame, L.P., Universite de Abobo-Adjame, 02 BP 801 Abidjan (Cote de'Ivoire). Laboratoire de Biochimie et Technologie des Aliments de l'Unite de Formation et de Recherche des Sciences et Technologies des Aliments | Zoro, Bi, I.A., Universite d'Abobo-Adjame, 02 BP801 Abidjan (Cote d'Ivoire). Laboratoire de Genetique de l'Unite de Formation et de Recherche des Sciences de la Nature
Four non-specific acid phosphatases designated as BLsAP1, BLsAP2, RLsAP1, and RLsAP2 have been previously isolated from edible seeds of Lagenaria siceraria, an oleaginous cucurbit categorized as a neglected or an orphan crop. In this study, the phosphotransferase activity of these biocatalysts for further biotechnological and industrial applications were examined. Investigations revealed that each phosphatase showed ability to catalyze phosphate ester synthesis uing pyrophosphate as the phosphate group donor and phenol as acceptor. To increase synthesis rates and yields of transfer product, the optimum conditions of transphosphorylation reactions were investigated. It was shown that transfer yields were highly dependent on pH, reaction period, donor and acceptor concentrations and enzyme amount. Shorter time course reactions (1.5 h at the most) were observed for optimum synthesis, suggesting energy saving for industrial processes. In experimental optimum conditions, average yields of 79.4 + or - 0.5, 58.2 + or - 0.6, 67.7 + or - 0.5 and 62.4 + or - 0.2% were obtained for reactions catalyzed by non-specific acid phosphatases BLsAP1, BLsAP2, RLsAP1 and RLsAP2, respectively. Considering these interesting phosphotransferase properties, it is likely that acid phosphatases from L. siceraria seeds would be useful to phosphorylate a wide range of nucleophile compounds. Thus, these biocatalysts could be considered as new valuable tools for bioindustrial applications.
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