Sequence analyses of the coconut enolase 3' end reveals a CACTG motif found within the 3'-untranslated region
2011
Cueno, M.E. | Laude, R.P. | Laurena, A.C.
As the coconut drupe matures, medium-chain fatty acids accumulate suggesting de novo fatty acid synthesis is active until drupe maturation is reached. This suggests that an active transcription of enolase mRNA takes place. Detection of coconut enolase transcripts from 4-5, 5-6, 6-7 and 7-8 month old coconut endosperms were made through 3' RACE. PCR products were cloned and sequence alignment showed higher homology between coconut and palm kernel enolose 3' CDS compared with that of rice and corn. Sequence alignment between the coconut and rice enolose 3' UTR revealed a common CACTG motif which is known to be conserved among the CACTA transposon family and has been speculated to insert regulatory elements. Folding pattern prediction of the coconut enolose 3' UTR revealed the occurrence of folding located immediately after the CACTG motif. We speculated this region to be the binding-site of regulatory elements.
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