Human umbilical cord blood serum, a better alternative of fetal bovine serum
2013
Ahmad, A. (Punjab Univ., Lahore (Pakistan). School of Biological Sciences) | Umer, R. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Inst. of Biochemistry and Biotechnology) | Shakoori, A.R. (Punjab Univ., Lahore (Pakistan). School of Biological Sciences)
Serum is essential component of cellular growth medium and consists of complex mixture of low and high molecular weight biomolecules required for cellular growth and maintenance. Fetal bovine serum (FBS) is extensively used in cell culture medium and its requirement is on the increase. Several alternatives of FBS in the form of goat, horse and human serum were tested but failed. Chemically defined medium are also in the market but they are very expensive and do not support growth as efficiently. There are also many scientific and ethical problems with the use of FBS so there is a need for an alternative which should have no ethical and scientific problems. Present study was conducted to test cord blood serum (CBS) as an alternative of FBS. HeLa, rMSC, hMSC, HBC-1 and HBC-3 were grown in the presence of 5, 10 and 15% FBS and CBS for 48 h. All cell lines were grown in the presence of 10% FBS and CBS for 0 to 120 h to calculate doubling time. HeLa, rMSC, and hMSC showed better growth and proliferation in the presence of CBS as against HBC-1 and HBC-3 in the presence of FBS. CBS also resulted in better attachment of HeLa and rMSC in short time. Doubling time of HeLa, rMSC and hMSC was reduced in the presence of CBS, while increased in the case of HBC-land HBC-3. This study proves that CBS can be a better alternative to FBS as it accelerates the proliferation of mesenchymal stem cells as well as HeLa cells and also helps the cells to adhere better CBS, has therefore, a great potential to be used for cell culture, to humanize the products used for cellular therapy and regenerative medicine.
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Эту запись предоставил National Agricultural Research Centre