Blueberry red ringspot virus Eliminated from Highbush Blueberry by Shoot Tip Culture
Spak, J., Academy of Sciences of the Czech Republic, Ceske Budejovice (Czech Republic). Inst. of Plant Molecular Biology | Pavingerova, D., Academy of Sciences of the Czech Republic, Ceske Budejovice (Czech Republic). Inst. of Plant Molecular Biology | Pribylova, J., Academy of Sciences of the Czech Republic, Ceske Budejovice (Czech Republic). Inst. of Plant Molecular Biology | Spakova, V., Academy of Sciences of the Czech Republic, Ceske Budejovice (Czech Republic). Inst. of Plant Molecular Biology | Paprstein, F., Research and Breeding Institute of Pomology, Holovousy (Czech Republic) | Sedlak, J., Research and Breeding Institute of Pomology, Holovousy (Czech Republic)
In order to find an effective elimination method of Blueberry red ringspot virus (BRRV), shoot tips of 2-3 mm size originating from buds of highbush blueberry cv. Darrow naturally infected by BRRV and collected in winter months were regenerated and multiplied on woody-plant medium (WPM) with 1.5 mg/l zeatin and 20 g/l sucrose. Developed shoots tested negative by polymerase chain reaction (PCR) for BRRV were transferred onto a rooting medium consisting of WPM supplemented with 1 mg/l indole-3-butyric acid. Rooting plants were transferred into soil and tested twice by PCR 6 months apart. Ninety per cent eradication of BRRV was achieved while isolating shoot tips, multiplying them in one vegetative generation, and regenerating whole 50-60 cm high plants within 30 months. All plants developed rich fruits after their overwintering in a cold greenhouse and were tested BRRV negative in summer 2013.
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