Ketahanan tiga genotipe cabai terhadap infeksi dua isolat chilli veinal mottle potyvirus
2012
Hidayat S.H.
Many factors influence virus replication and movement in the plant cell, among others are virulence of the virus and resistance of the infected plants. Infection of two ChiVMV isolates on three chilli pepper genotypes were assayed to observe disease development. The study was conducted in Cikabayan screen house and Laboratory of Plant Virology, Bogor Agricultural University from April to September 2008. Three genotypes of chilli pepper with different resistance to ChiVMV were selected for this study, i.e. IPB C521 (highly resistant), IPB C17 (resistant), and IPB C99 (highly susceptible). Each of the genotypes was mechanically inoculated separately with virulent isolate (ChiVMV CKB) and mild isolate (ChiVMV BL) of the virus. Disease incidence and incubation period of the virus were observed based on symptom development, whereas virus titer and trans location were detected using dot immunobinding assay (DIBA). Disease incidence caused by infection of ChiVMV CKB and ChiVMV BL reached 100 percent on genotype IPB C99, but no disease incidence was observed on genotype IPB C521. Incubation period of ChiVMV CKB was relatively shorter (7 to 9 days) than those of ChiVMV BL (9 to 14 days). Positive reaction on DIBA was strongly shown for ChiVMV CKB with a high score of virus titer (5 to 6) and sensitivity up to dilution factor of 1:10 the power of 6. This study shows that chiVMV CKB has the ability to cause more severe infection on chilli pepper than ChiVMV BL.
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