Effects of Tricholstatin A on in Vitro Development of Porcine Parthenogenetic and Nuclear Transfer Embryos
2013
Diao, Y.F., Chungnam National University, Daejeon, Republic of Korea | Kenji, N., Chungnam National University, Daejeon, Republic of Korea | Han, R.X., Chungnam National University, Daejeon, Republic of Korea | Lin, T., Chungnam National University, Daejeon, Republic of Korea | K, R., Chungnam National University, Daejeon, Republic of Korea | Oqani,, Chungnam National University, Daejeon, Republic of Korea | Kang, J.W., Chungnam National University, Daejeon, Republic of Korea | Jin, D.I., Chungnam National University, Daejeon, Republic of Korea
Development potential of cloned embryos is related closely to epigenetic modification of somatic cell genome. The present study was to investigate the effects of applying histone deacetylation inhibitor, trichostatin A to activated porcine embryos on subsequent development of porcine parthenogenetic and nuclear transfer embroys. Electrically activated oocytes were treated with 5 nM TSA for different exposure times and then the activated embryos were cultured for 7 days.
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