Generation and Evaluation of High b-Glucan Producing mutant Strains of Sparassis crispa
2013
Kim, S.R., Gyeongsang National University, jinju, Republic of korea | Kang, H.W., Gyeongsang National University, jinju, Republic of korea | Ro, H.S., Gyeongsang National University, jinju, Republic of korea
A chemical mutagenesis technique was employed for development of mutant strains of Sparassis crispa targeting the shortened cultivation time and the high b-glucan content. The homogenized mycelial fragments of S. crispa IUM4010 strain were treated with 0.2 vol% nethyl methanesulfonate, an alkylating agent, yielding 199 mutant strains. Subsequent screening in terms of growth and b-glucan content yielded two mutant strains, B4 and S7. Both mutants exhibited a significant increase in b-glucan productivity by producing 0.254 and 0.236 mg soluble b-glucan/mg dry cell weight for the B4 and S7 strains, respectively, whereas the wild type strain produced 0.102mg soluble b-glucan/mg dry cell weight. The results demonstrate the usefulness of chemical mutagenesis for generation of mutant mushroom strains.
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