Crop proteomics: Practical method for high resolution of two-dimensional electrophoresis
2012
Kim, U.G., Pusan National University, Miryang, Republic of Korea | Jung, H.J., Pusan National University, Miryang, Republic of Korea | Lee, S.J., Pusan National University, Miryang, Republic of Korea | Kim, S.T., Pusan National University, Miryang, Republic of Korea
Two-dimensional gel electrophoresis (2-DGE) is one of the most important technologies for high-resolution separation of proteins for proteomics. In this study, we present a detail 2-DGE protocol which allows detection and quantification of total plant proteins separated on gels to improve matching in image analysis. This protocol high-lighted here may be useful for researchers, who like to first study for the development of protein biomarkers involved in development, biotic and abiotic stresses in plant.
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