Physicochemical Properties and Biological Activities of Beauveria bassiana Cultured Medium
2013
Lee, E.J., Dankook University, Cheonans, Republic of Korea | Jang, K.H., Dankook University, Cheonans, Republic of Korea | Lim, S.Y., Dankook University, Cheonans, Republic of Korea | Kim, J.J., RDA, Suwon, Republic of korea | Kim, K., Suwon University, Suwon, Republic of korea | Hong, S.I., Korye Bio., Hwasung, Republic of Korea | Lee, D.J., Dankook University, Cheonans, Republic of Korea
The present study was conducted to estimate the antioxidant activities, physicochemical properties and biological activities in Beauveria bassiana. Glucose was the most abundant in B. bassiana (15.2 g·L−1) of 3 day medium by the HPLC analysis of free sugar. Citric acid were observed the highest components in B. bassiana (276.4 mg·L−1) of 3 day medium by the HPLC analysis of organic acid. The composition of Glutamate was the highest among amino acids in B. bassiana (987.0 pmol·μl−1) of 3 day medium. Na was the highest among minerals. It was focused on antioxidant and anticancer activities of B. bassiana. The highest contents of total polyphenol (6.51 ± 0.04 ppm) and flavonoid (1.10 ± 0.06 ppm) were detected in the B. bassiana of 15 day medium. The IC50 value of DPPH radical scavenging activity was ranged from 263.4 ± 13.3 to 461.9 ± 23.9 μg·ml−1 SOD activity was ranged from 93.7 ± 16.6 to 99.8 ± 3.8 μg·ml−1. Also ABTS radical activity was ranged from 99.7 ± 1.2 to 156.9 ± 2.9 μg·ml−1 of which the strongest activity was determined of 15 day medium. Especially, 93.7 ± 16.6 μg·ml−1 of 15 day medium showed highest activity by SOD. In cytotoxicity test against three cancer cell lines, HepG2 (liver), MCF-7 (breast) and A549 (lung), showed the highest toxicity with IC50 values of HepG2 (127.8 ± 2.3 μg·ml−1) cell lines of 10 day medium. Also MCF-7 (129.1 ± 6.8 μg·ml−1), A549 (163.8 ± 4.1 μg·ml−1) showed the highest toxicity of 5 day medium. These data suggest Enthomopathogenic of B. bassiana culture medium could be potential candidate for natural antioxidants.
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