Modification of the commercial silkworm eggs adequate for silkworm transgenesis
2013
Kim, S.W., RDA, Suwon, Republic of Korea | Kang, M.U., The Foundation of Agricultural Technology Commercialization and Transfer, Suwon, Republic of Korea | Kang, S.W., RDA, Suwon, Republic of Korea | Yun, E.Y., RDA, Suwon, Republic of Korea | Choi, K.H., RDA, Suwon, Republic of Korea | Kim, S.R., RDA, Suwon, Republic of Korea | Park, S.W., Catholic University of Daegu, Daegu, Republic of Korea | Nho, S.K., Catholic University of Daegu, Daegu, Republic of Korea | Goo, T.W., RDA, Suwon, Republic of Korea
Silkworm transgenesis scientists have done some genetic modification work on multivoltine silkworms, but that type of silkworms is less commercial feasible. They are easy to manipulate, because they breed all year round. But the commercial silkworm variety must undergo hydrochloric acid treatment at a high temperature to be artificially hatched. Hydrochloric acid penetrates through the holes in the silkworm eggs, fatally damaging their reproduction. So it had been thought that altering the properties of the commercial silkworm variety would be very difficult. So we have tried to make from diapause to non-diapause eggs using diapauses varieties, Backokjam and Jam 124. At present, our group has establishing the conditions for non-diapause eggs. Oviposited eggs after 40 ~ 60 hours were incubated for 24 hours at 15 ~ 20oC with dark condition. Non-diapause eggs were completely induced. The hatching rate, molting rate and pupation rate of non-diapause Jam 124 and Backokjam eggs showed no differences compared to diapause eggs. When transgenic silkworm using the non-diapause eggs, the hatching rate showed that non-diapause eggs induced from diapause were 40 ~ 70%, diapause eggs treated with artificial incubation were 10 ~ 30%, and polyvoltine strains, HM eggs were 30 ~ 50%. Therefore, we suggest that modification techniques of the commercial silkworm eggs adequate for silkworm transgenesis can be used to develop transgenic silkworms more easily.
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