Determination of Eleutherosides and β-Glucan Content from Different Parts and Cultivating Areas of A. senticosus and A. koreanum
2013
Kim, Y.H., Kangwon National University, Gangwon, Republic of Korea | Bae, D.B., Kangwon National University, Gangwon, Republic of Korea | Lee, J.S., Kangwon National University, Gangwon, Republic of Korea | Park, S.O., STR Biotech Co., LTD, Gangwon, Republic of Korea | Lee, S.J., STR Biotech Co., LTD, Gangwon, Republic of Korea | Cho, O.H., University of Massachusetts Medical School, Worcester, USA | Lee, O.H., Kangwon National University, Gangwon, Republic of Korea
The present study was performed to quantitatively analyze eleutherosides (B and E) and β-glucan in different plant parts of three cultivars (Chungnam, Gangwon, and Jeju) of Acanthopanax senticosus and Acanthopanax koreanum using HPLC and a commercial enzyme kit. Our results showed high linearity in the calibration curves as the coefficients of correlation (R2) were 0.998 (eleutheroside B) and 0.999 (eleutheroside E), respectively. Eleutheroside B and E were found in stem extracts of A. koreanum cultivated in Jeju (1,122 μg/g, eleutheroside B) and A. senticosus cultivated in Chungnam (2,536 μg/g, eleutheroside E), respectively. However, eleutheroside B was not detected in any part of A. senticosus cultivated in Chungnam. For β-glucan contents, stems of A. senticosus and A. koreanum showed higher than other parts. Furthermore, the β-glucan content in stems of A. koreanum cultivated in Gangwon was significantly higher than in those of other cultivars. These results show that the contents of eleutheroside B, E, and β-glucan were higher in stem extracts of A. senticosus and A. koreanum than other parts. Moreover, our results suggest that the contents of eleutheroside B, E, and β-glucan in A. senticosus and A. koreanum are influenced by cultivation area and the selected part.
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