The Nuclear DNA Content and Histological Characteristics of Triploid Poplars inVitro
2013
Bae, E.K., Korea Forest Research Institute, Suwon, Republic of Korea | Lee, H.S., Korea Forest Research Institute, Suwon, Republic of Korea | Lee, J.S., Korea Forest Research Institute, Suwon, Republic of Korea | Choi, Y.I., Korea Forest Research Institute, Suwon, Republic of Korea | Park, S.Y., Chungbuk National University, Cheonju, Republic of korea
Herein we analyzed the nuclear DNA content and the histological characteristics of the triploid of the Hyunsasi (Populus alba × P. glandulosa F1) which were developed for biomass production and molecular breeding research. The flow cytometric analysis showed that the nuclear DNA content of the 3 triploids were 1.6 times greater than those of the diploid. In terms of histological characteristics, the cross-section area of the stem of Line-18 was 1.6 times larger than that of the diploid. The area of pith, and cortex and phloem of the stem of Line-18 was also 1.6 and 2.0 times larger than that of the diploid, respectively. Moreover, the length and area of guard cell of Line-18 was 1.2 times larger than that of the diploid. These results helps to understand the cytological characteristics of the triploid poplar clones, and further investigations in the growth rate and wood properties of the triploids growing in the field will determine whether the triploid poplars are good candidates for molecular breeding programs and for the improvement of industrial biomass productivity.
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