Inhibitory Effects of S-Allylmercaptocysteine Derived from Aged Garlic on Cholesterol Biosynthesis in Hepatocytes
2013
Yang, S.T., Kyungsung University, Busan, Republic of Korea
The present study was undertaken to elucidate the mechanisms underlying the cholesterol-lowering effect of S-allylmercaptocysteine (SAMC) derived from aged garlic. Rat hepotocytes and HepG2 cells were used to determine the short-term effects of SAMC on [14C] acetate incorporation into cholesterol, and several enzymatic steps. The cells were grown in Dulbecco modified Eagle medium supplemented with 10% fetal bovine serum and treated with 20, 40, 60 and 80 μg/ml of SAMC. At concentration of 20~40 μg/ml, no significant cells viability effect was noted during those incubation periods. However, at a concentration 60 μg/ml, cell viability decreased approximately 50% compared with the control. The treatment of cells with 5, 10, 15, and 20 μg/ml of SAMC resulted in a marked of [14C]-acetate incorporation into cholesterol. At concentration of 15 μg/ml, the cholesterol synthesis was inhibited 79% in cells. The activities of lipogenic enzymes, fatty acid synthase (FAS), and glucose-6-phosphate dehydrogenase (G3PDH) were measured in culture hepatocytes treated with the inhibitors. The activity of FAS in cells treated with 0.95 nmol SAMC was 19% lower than that of nontreated cells, and no affected G6PDH activity, 3- hydroxy-3-methylglutaryl Co A activity was decreased at concentration dependant manner. The present study demonstrates that SAMC is effective in inhibiting cholesterol biosynthesis.
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