Neuroprotective effects of resveratrol on 6-hydroxydopamine-induced damage of SH-SY5Y cell line
2014
Chang, G.C., Kangwon National University, Chuncheon, Republic of Kroea | Kim, H.C., Kangwon National University, Chuncheon, Republic of Kroea | Wie, M.B., Kangwon National University, Chuncheon, Republic of Kroea
Parkinson is disease is known to exhibit progressive degeneration of the dopaminergic neurons in the substantia nigra via inhibition of glutathione metabolism. It is well known that 6-Hydroxydopamine (6-OHDA) induces Parkinson is disease-like symptoms, while resveratrol (3,5,4-trihydroxystilbene) has been shown to have anti-inflammatory and antioxidant effects. In the present study, we investigated the neuroprotective effects of resveratrol, a phytoalexin found in grapes and various plants, on 6-OHDA-induced cell damage to the SH-SY5Y human neuroblastoma cell line. Resveratrol (5 and 10§) inhibited 6-OHDA (60 §)-induced cytotoxicity in SH-SY5Y cells and induced a reduction of the number of apoptotic nuclei caused by 6-OHDA treatment. Additionally, the total apoptotic rate of cells treated with both resveratrol (10 §) and 6-OHDA (60§) was less than that of 6-OHDA treated cells. Resveratrol also dose-dependently (1, 5 and 10 §) scavenged reactive oxygen species (ROS) induced by 6-OHDA in SH-SY5Y cells and prevented depletion of glutathione in response to the 6-OHDA-induced cytotoxicity in the glutathione assay. Overall, these results indicate that resveratrol exerts a neuroprotective effect against 6-OHDA-induced cytotoxicity of SH-SY5Y cells by scavenging ROS and preserving glutathione.
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