The Effects of Bee Venom on Tumor Necrosis Factor (TNF)- ¥� Induced Inflammatory Human HaCaT Keratinocytes
2014
Lee, W.R., Catholic University of Daegu, Daegu,Republic of Korea | Kim, K.H., Catholic University of Daegu, Daegu,Republic of Korea | An, H.J., Catholic University of Daegu, Daegu,Republic of Korea | Kim, J.Y., Catholic University of Daegu, Daegu,Republic of Korea | Han, S.M., National Academy of Agricultural Science and Technology, Jeonju, Republic of Korea | Lee, K.G., National Academy of Agricultural Science and Technology, Jeonju, Republic of Korea | Park, K.K., Catholic University of Daegu, Daegu,Republic of Korea
Bee venom (BV) therapy has been used as a traditional medicine to treat a variety of conditions, such as arthritis, back pain, cancerous tumors, and skin diseases. However, regulatory effects of BV on tumor necrosis factor (TNF)- ¥� -induced HaCaT cell migration or anti-inflammatory have not been explored. In the present study, we investigated the effects of BV on HaCaT cell migration and anti-inflammation. HaCaT cell migration was evaluated by wound-healing assay. The pro-inflammatory cytokines such as TNF- ¥� , interleukin (IL)- 1¥� , and IL-8 were examined by ELISA or Western blotting. BV treatment led to an increase in migration of HaCaT cells for 24 and 48 h. Especially, 10 ng/ml of BV were significantly increased HaCaT cell migration. Also, BV suppressed the secretion of TNF- ¥� , IL- 1¥� , and IL-8 in culture medium with HaCaT cells. In addition, Western blot results demonstrate that BV suppressed the expression of TNF- ¥� and IL- 1¥� , in HaCaT cells. Especially, 1 or 10 ng/ml of BV markedly decreased the expression of pro-inflammatory cytokines. These results demonstrate the potential of BV for the prevention of skin inflammation induced by TNF- ¥� .
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