Determination of Soluble Carbohydrates in Soybean Seeds Using High Performance Liquid Chromatography with Evaporative Light Scattering Detection
2014
Kim, G.H., Kangwon National University, Chuncheon, Republic of Kroea | Hwng, Y.S., Kangwon National University, Chuncheon, Republic of Kroea | Ahn, K.G., Kangwon National University, Chuncheon, Republic of Kroea | Kim, G.P., Kangwon National University, Chuncheon, Republic of Kroea | Kim, M.J., Kangwon National University, Chuncheon, Republic of Kroea | Hong, S.B., Kangwon National University, Chuncheon, Republic of Kroea | Moon, J.K., Rural Development Administration, Jeonju, Republic of Korea | Choung, M.G., Kangwon National University, Chuncheon, Republic of Kroea
In the present study, a new analytical method was devised for the simultaneous determination of soluble carbohydrates in soybean seeds using high performance liquid chromatography/evaporative light scattering detection (HPLC/ELSD). The limit of quantification (LOQ) for soybean soluble carbohydrates ranged from 5.6¢¦7.6 mg/kg using the HPLC/ELSD method and from 16.2¢¦33.9 mg/kg using the high performance liquid chromatography/refractive index detection (HPLC/RID) method. Therefore, the HPLC/ELSD method was more sensitive than HPLC/RID. The precision values for retention time and peak area of the HPLC/ELSD method were evaluated by inter-day (n=5) and intra-day (n=10) assays using a standard solution. All precision values (CV2.5%) for soybean soluble carbohydrates were acceptable and fulfilled international acceptance criteria. All linear calibration curves were obtained with a correlation coefficient of R¡�20.999. The contents of soluble carbohydrates for the ""Shingikong"" (yellow soybean) and ""Cheongjakong 3"" (black soybean) samples were analyzed using the HPLC/RID and HPLC/ELSD methods. The difference in carbohydrate contents between the two detection methods was significant. Carbohydrate contents in the HPLC/ELSD method were higher than those in the HPLC/RID method. Overall, the HPLC/ELSD method showed satisfactory resolution with a favorable LOQ and reproducibility. Therefore, these results indicate that the HPLC/ELSD method may be applied to determine the contents of soluble carbohydrates in soybean seeds and related food stuffs.
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