Phenotypic and molecular characteristics of second clone (T0V2) plants of the LeLs-antisense gene-transgenic chrysanthemum line exhibiting non-branching
2013
Lee, S.Y., National Institute of Horticultural and Herbal Science, Suwon, Republic of Korea | Kim, J.H., National Institute of Horticultural and Herbal Science, Suwon, Republic of Korea | Cheon, K.S., National Institute of Horticultural and Herbal Science, Suwon, Republic of Korea | Lee, E.K., National Institute of Horticultural and Herbal Science, Suwon, Republic of Korea | Kim, W.H., National Institute of Horticultural and Herbal Science, Suwon, Republic of Korea | Kwon, O.H., National Institute of Horticultural and Herbal Science, Suwon, Republic of Korea | Lee, H.J., National Institute of Horticultural and Herbal Science, Suwon, Republic of Korea
This study examined the phenotypic and molecular characteristics of the 2nd clone (T0V2) plants of LeLs-antisense gene-transgenic chrysanthemum line (LeLs80) that exhibited non-branching, proving the relevance of these characteristics as a factor for use in environmental risk assessment. Results of the Southern blot analysis showed that three copies of the LeLs-antisense gene were introduced into the transgenic line, and northern analysis showed that the transcripted gene was normally expressed in the transgenic line. A flanking T-DNA sequencing method was used to determine that sequences of 184 and 464 bps flanked the LeLs-antisense gene in the transgenic line. These sequences, respectively, matched the 35S promoter for expression of the nptⅡ gene and the NOS terminator for expression of the LeLs-antisense gene within the pCAMBIA 2300 vector.
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