In vitro anthelmintic activity of some Mediterranean plants against Haemonchus contortus infective stage

A., Aïssa; F., Manolaraki; E., Pardo; I.A., Znaïdi; H., Ben Salem; K., Kraiem; H., Hoste

In vitro anthelmintic activity of some Mediterranean plants against Haemonchus contortus infective stage

2013

The use of bioactive tanniniferous plants seems a promising alternative to control infections parasitic nematodes of the gastro intestinal tract in small ruminants. Both in vitro and in vivo studies, have confirmed the anthelmintic properties of several forage legumes, or tropical and browse plants. The aim of the present study was to evaluate the in vitro anthelmintic (AH) effect of 3 Tunisian plants (Trigonella foenum graecum, Periploca angustifolia Labill., Ceratonia siliqua) on Haemonchus contortus infective larvae. The larval exsheathment inhibition assay (LEI) was used to determine the potential inhibitory effects of 5 plant extracts at different concentrations (1200, 600, 300, 150μg per ml). The inhibition effect at the highest concentration ranged between 82.97-100 per cent for C. siliqua (leaves), P. angustifolia (pods and leaves), T. foenum graecum (whole plant) and at 53.5 per cent for C. siliqua (fruits). The possible implication of polyphenols and per or tannins in the anthelmintic activity was showed after measuring the total phenols, total tannins and the biological activity of the extracts tested.

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