Morphogenesis of Prunus regenerants in vitro with using of 2,5-olygoadenylates at the micro propagation stage | Морфогенез растений-регенерантов рода Prunus в культуре in vitro на этапе микро размножения с использованием 2,5-олигоаделилатов
2013
Krasinskaya, T.A. | Kukharchik, N.V., National Academy of Sciences. Scientific and Practical Center for Potato, Vegetable and Fruit Growing (Belarus). Institute for Fruit Growing | Kulak, T.I., National Academy of Sciences, Minsk (Belarus). Institute of Bioorganic Chemistry
Several researches of the influence of 2,5-olygoadenylates on agricultural plants prove the increase of morphological and physiological characteristics of plants' development, increment of the crop yield and pest resistance of the mentioned plants. But along with this, 2,5-olygoadenylates possess high phyto- toxicity and their use during plant cultivation in vitro may lead to loss of planted explants up to 94,7 %. Therefore, the research of the influence of 2,5-olygoadenylates on the development of meristem of Prunus regenerants on the micro propagation stage appears an important scientific problem. It is established that Trimer 2 – analog 2,5-olygoadenylate with 9-(2,3-angidro-B-D-ribophyranosyl)adenin fragment – is more phytotoxic for regenerants of cv. Novodvorskaya at the first contact with nutrition medium than Trimer 1 (analog of 2,5-olygoadenylate with virazol fragment). Concentrations 10-8 M and 10-7 M of Trimer 2 and 10-7 M of trimer 1 are optimal for normal plant development and maximum micro propagation rate from one meristem. The maximum micro propagation rate is noted on the medium with Trimer 1 at the concentration 10-7 M and with Trimer 2 at the concentration 10-8 M and 10-7 M for cv. Izmailivsky regenerants at the end of 5-th subculture. It is recommended to use Trimer 2 at the concentration of 10-8 M and 10-7 M for effective production of Prunus lants in culture in vitro.
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