Characterization of PR-10 gene derived from highly resistant 93 - 3 - 98 pear inoculated with scab (Venturia nashicola)
2015
Chun, J.A., Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Kim, S.H., Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Cho, K.H., Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Kim, D.H., Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Choi, I.M., Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Shin, I.S., Pear Research Station, National Institute of Horticultural and Herbal Science, RDA, Naju, Republic of Korea
A PyrcpPR-10 gene with differentially expressed was isolated by using the suppression subtractive hybridization assay between 93 - 3 - 98 (highly resistant against scab caused by Venturia nashicola) and Sweat Skin (highly susceptible) and analyzed the expression pattern according to organs and cultivars. The full length of PyrcpPR-10 was cloned as 743bp with 480bp s ORP, and was determined to encode a protein of 159 amino acid residues. On analyzing PyrcpPR-10 gene sequence compared with resistant and susceptible cultivars, Hwangsilri (resistant), Gamcheonbae (moderately resistant), Wonhwang (moderately susceptible), Niitaka (highly susceptible), and Sweat Skin (highly susceptible) had identical gene sequence but Bartlett (highly resistant) showed partly different sequences. The deduced amino acid sequence showed 64 - 98% homology and had the GXGGXG motif to known amino acid of other plants PR-10 by the BLAST x analysis. Among several organs or tissues, petal was showed highest expression level of PyrcpPR-10 gene followed by leaf, floral axis, bud, and bark. The expression level of PyrcpPR-10 gene was dramatically increased at 24 hr after inoculation in all cultivars and also up-regulated in accordance with resistant degree of cultivars. While resistant cultivars ( Bartlett, 93 - 3 - 98 , and Hwangsilri ) induced relatively high expression level of PyrcpPR-10 gene, susceptible cultivars ( Niitaka , and Sweat Skin ) showed low expression level. PyrcpPR-10 gene is assumed that it is directly connected with defense mechanisms to pear scab.
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