Identification of Korean Poaceae Weeds Based on DNA Sequences
2015
Lee, J., National Academy of Agricultural Science, RDA, Wanju, Republic of Korea | Kim, C.S., National Academy of Agricultural Science, RDA, Wanju, Republic of Korea | Lee, I.Y., National Academy of Agricultural Science, RDA, Wanju, Republic of Korea | Oh, H.J., National Academy of Agricultural Science, RDA, Wanju, Republic of Korea | Kim, J.H., National Institute of Biological Resources, Inchon, Republic of Korea | Kim, S.Y., National Institute of Biological Resources, Inchon, Republic of Korea
Korean Poaceae includes approximately 80 species of the agricultural weeds. Precise species identification is the first step for more effective weed management in the agricultural fields. However, the identification of species in Poaceae is not easy without the assistance of taxonomists or identification experts although they are relatively easy to distinguish from the plants of the other family by the unique characteristics of caryopsis. Thus, DNA barcode was suggested as an alternative powerful technique for species identification by using short sections of DNA from a specific region of the genome. Two standard barcode markers of vascular plants, chloroplast rbcL and matK, and a supplementary nuclear ribosomal Internal Transcribed Spacer (ITS) region were used for barcode of major Korean Poaceae weeds, 403 individuals of 84 taxa. All the barcode markers revealed a good level of sequencing success with the lowest 73.7% for matK and the highest 88.8% for rbcL. The barcode sequences were deposited to the National Center for Biotechnology Information (NCBI) database for public use. Combined matK and ITS showed very high resolving power with 92.9%. Besides the identification of weeds for weed managment, the generated DNA barcode data could be used for many other applications such as rapid biodiversity assessment and conservation prioritization.
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