In vitro propagation of Gypsophila paniculata L. through plant tissue culture techniques

Apical meristem as explant was used to micropropagate the economically important cut flower plant, Gypsophila paniculata L. by using in vitro techniques. Different media were used for plant propagation, and multiple shoots were obtained on MS medium fortified with different concentrations of BAP along with various combinations of NAA and Kin. After removing the leaves, apical meristems were washed with tap water and household detergent and also dipped in commercial sodium hypochlorite (bleach) for sterilization. Then these sterilized plants are inoculated on MS medium supplemented with different plant growth regulators like BAP (at concentrations of 0.25, 0.5, 1.0, 1.5 & 2.0 mg/l), for multiplication, different concentrations of BAP and NAA, combinations of BAP and Kin (mg/l), BAP1 + Kin (mg/l) combinations of BAP1, Kin and NAA (mg/l) were used, for rooting different concentrations of NAA (0.1, 0.2, 0.3, 0.4 & 0.5 mg/l) were used. The optimum temperature for plantlets formation in culture room was maintained at 22 plus minus 2 degree C. The cultures were incubated at 16 hours of light period with light intensity of 2000-3000 lux. All media were based on the MS basic salts, (macro & micro) iron EDTA, vitamins, sucrose 30g/l and 0.1g of myoinasitol. The pH of media was adjusted at 5.57, and the cultures containing 1.5g/l of phytagel before autoclaving at 121 degree C degree 15lb/inch2 for 20 minutes. Among all the media tested the highest number of shoot emergence, multiple shoot formation, and maximum shoot length was obtained in medium supplemented with BAP 1mg/l after 4 days of inoculation. Which shows the maximum shoot length of 6.53 (cm) with 86 percentage response observed in same medium after 21 days. For root initiation the best medium was NAA 0.5 mg/l which showed 85 % of root initiation with length of 2.66 (cm) after 11 days of inoculation. A completely randomized design was used for the experiment with three replicates. The data was analyzed by COSTAT V.63: statistical software (Cohort software, Berkely, California) and Duncan' New Multiple Range.